Abstract
The effect of selected cations on DNA synthesis by DNA-polymerase of avian myeloblastosis virus (AMV) was studied. Zinc ions at low concentration (0.2mM) in the assay system enhanced the activity about 2 x fold and at higher concentration (2.0 mM) inhibited the activity completely. In contrast, addition of lithium and potassium salts produced inhibitory effects in this ionic concentration range. Replacement of K+ ion had an inhibitory effect on the activity.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Avian Leukosis Virus / enzymology*
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Avian Myeloblastosis Virus / enzymology*
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DNA / biosynthesis
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DNA-Directed DNA Polymerase / metabolism*
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Enzyme Activation
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Lithium / pharmacology
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Nucleic Acid Synthesis Inhibitors
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Potassium / pharmacology
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Zinc / administration & dosage
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Zinc / pharmacology*
Substances
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Nucleic Acid Synthesis Inhibitors
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DNA
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Lithium
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DNA-Directed DNA Polymerase
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Zinc
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Potassium