HMGN5: a potential oncogene in gliomas

J Neurooncol. 2011 Sep;104(3):729-36. doi: 10.1007/s11060-011-0558-9. Epub 2011 Mar 4.

Abstract

Gliomas are the most common primary brain tumors in the central nervous system and a leading cause of tumor-related death. High-mobility group nucleosome binding domain 5 (HMGN5/NSBP1), which is highly expressed in breast cancer and in hormone-induced mouse uterine adenocarcinoma, acts as a potential oncogene in gliomas. In this study, the role of HMGN5 in the proliferation of human glioma cells was investigated by lentivirus-mediated RNA interference (RNAi). The decrease in HMGN5 expression in human glioma U251 and U87 cells caused cell cycle arrest in the G1 phase and a delay in cell proliferation, as well as resulting in more apoptosis and an inhibition of clonogenic growth in soft agar in U251 cells; these results suggest that HMGN5 is required for tumorigenesis in vitro. Furthermore, HMGN5 was highly expressed in both high-grade and low-grade glioma tissue samples compared with normal brain tissues. Collectively, our data suggest that HMGN5 may play a critical role in the development of gliomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Apoptosis / genetics
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / metabolism*
  • Cell Cycle
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Transformation, Neoplastic / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Glioma / genetics*
  • Glioma / metabolism*
  • HMGN Proteins / genetics*
  • HMGN Proteins / metabolism
  • Humans
  • Integrin alpha1 / metabolism
  • Male
  • Middle Aged
  • RNA, Messenger / metabolism
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Time Factors
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transfection / methods
  • Young Adult

Substances

  • HMGN Proteins
  • HMGN5 protein, human
  • Integrin alpha1
  • RNA, Messenger
  • RNA, Small Interfering
  • Trans-Activators