Isotypic neutralizing antibodies against mouse GCP-2/CXCL6 inhibit melanoma growth and metastasis

Cancer Lett. 2011 Mar 1;302(1):54-62. doi: 10.1016/j.canlet.2010.12.013. Epub 2011 Jan 13.

Abstract

The chemokine granulocyte chemotactic protein (GCP)-2/CXCL6 promotes tumor growth as angiogenesis inducer and neutrophil chemoattractant. The neutralizing capacity and specificity of monoclonal mouse anti-murine (mu)GCP-2/CXCL6 antibodies were evidenced by granulocyte chemotaxis and signaling assays. The half-life of the non-antigenic antibody in the blood circulation was approximately 15 days. The titers remained constant upon weekly injection. Tumor growth and lymphogenic metastases of human melanoma over-expressing muGCP-2 were reduced in mice treated with anti-muGCP-2. Moreover, the drop in muGCP-2 antibody titer correlated with the melanoma tumor size. Taken together, we show that functional blocking of GCP-2 inhibits tumor growth and metastases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacokinetics
  • Antibodies, Monoclonal / pharmacology
  • Antibodies, Neutralizing / immunology
  • Antibodies, Neutralizing / pharmacology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Chemokine CXCL6 / genetics
  • Chemokine CXCL6 / immunology*
  • Chemokine CXCL6 / metabolism
  • Chemotaxis, Leukocyte / drug effects
  • Female
  • Granulocytes / drug effects
  • Granulocytes / metabolism
  • Humans
  • Melanoma / genetics
  • Melanoma / pathology
  • Melanoma / prevention & control*
  • Melanoma, Experimental / genetics
  • Melanoma, Experimental / metabolism
  • Melanoma, Experimental / prevention & control
  • Mice
  • Mice, Inbred Strains
  • Mice, Nude
  • Neoplasm Metastasis
  • Xenograft Model Antitumor Assays

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neutralizing
  • Chemokine CXCL6