Inhibition of DNA excision by DNA polymerase-alpha inhibitor in UV-damaged HeLa cells

Toxicol Lett. 1990 Aug;52(3):253-9. doi: 10.1016/0378-4274(90)90034-j.

Abstract

HeLa cells treated with 10 J/m2 of ultraviolet (UV) radiation were examined for inhibition of DNA excision by inhibitors of DNA polymerase-alpha and -beta. DNA repair synthesis and excision were inhibited by aphidicolin, a specific inhibitor of DNA polymerase-alpha. Decreased release of radioactive nucleotides from UV-damaged DNA by the inhibitor indicates that the action of DNA polymerase-alpha activity is closely associated with DNA excision. Dideoxythymidine also inhibited DNA repair synthesis but failed to block the excision, suggesting that polymerization by DNA polymerase-beta might precede removal of damaged DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiotics, Antineoplastic / pharmacology*
  • Aphidicolin
  • Cells, Cultured
  • DNA Polymerase II / antagonists & inhibitors*
  • DNA Repair / drug effects*
  • Dideoxynucleosides / pharmacology*
  • Diterpenes / pharmacology*
  • HeLa Cells / drug effects*
  • HeLa Cells / enzymology
  • HeLa Cells / radiation effects
  • Humans
  • Ultraviolet Rays / adverse effects

Substances

  • Antibiotics, Antineoplastic
  • Dideoxynucleosides
  • Diterpenes
  • 2',3'-dideoxythymidine
  • Aphidicolin
  • DNA Polymerase II