[Lysophosphatidic acid acyltransferase β gene expression in newly diagnosed leukemia patients]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Dec;18(6):1422-6.
[Article in Chinese]

Abstract

This study was aimed to quantitatively detect the expression level of lysophosphatide acid acyltransferase β (lpaat β) mRNA in leukemia patients so as to provide theoretical basis for the target therapy of lpaat β in leukemia. Real-time fluorescently quantitative PCR was used to detect the relative expression level of lpaat β mRNA to analyze its expression change in various type of leukemia. The results showed that the lpaat β mRNA expression level in acute leukemia (AL) patients was significantly higher than that in normal controls (p < 0.05); lpaat β mRNA expression level in acute myeloid leukemia (AML) patients was significantly higher than that in normal controls (p < 0.05) and was positively correlated with white blood cell count (≥ 20.0 × 10(9)/L) (p < 0.05) and CD34 expression level of leukemia, but was not related with extramedullary infiltration. Except for acute promyelocytic leukemia (APL), the lpaat β mRNA expression level was negatively correlated with chemotherapy sensitivity in chronic myeloid leukemia (AML) patients. lpaat β mRNA expression level in chronic myeloid leukemia (CML) patients was significantly higher than that in normal controls (p < 0.05). lpaat β mRNA expression level in acute lymphoid leukemia (ALL) patients was not higher than that in normal controls (p > 0.05). It is concluded that the lpaat β gene overexpression exists in both AML and CML patients. lpaat β produced by AML cells probably plays an important role in abnormal proliferation and drug-resistance of AML cells.

Publication types

  • English Abstract

MeSH terms

  • Acyltransferases / genetics*
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Case-Control Studies
  • Child
  • Female
  • Gene Expression
  • Humans
  • Leukemia / genetics*
  • Leukemia / pathology
  • Male
  • Middle Aged
  • RNA, Messenger / genetics
  • Young Adult

Substances

  • RNA, Messenger
  • Acyltransferases
  • 2-acylglycerophosphate acyltransferase