Abstract
Retrovirus replication critically depends on a dynamic interplay between retroviral and host proteins. We report on the binding of the surface subunit (glycoprotein 120 (gp120)) of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) to the cytoplasmic C-terminus of the voltage-gated potassium channel BEC1 (brain-specific ether-a-go-go-like channel 1), an interaction that can result in the repression of BEC's activity and the inhibition of HIV-1 particle-release. BEC1 protein was found to be expressed in T cells and macrophages, the major target cells of HIV-1. Thus, gp120/BEC1 interaction may be involved in HIV-1 life cycle and/or pathogenesis.
Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Cell Line
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Chlorocebus aethiops
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Ether-A-Go-Go Potassium Channels / chemistry
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Ether-A-Go-Go Potassium Channels / genetics
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Ether-A-Go-Go Potassium Channels / metabolism*
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Gene Expression
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HIV Envelope Protein gp120 / chemistry
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HIV Envelope Protein gp120 / genetics
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HIV Envelope Protein gp120 / metabolism*
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HIV-1 / genetics
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HIV-1 / metabolism*
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HIV-1 / pathogenicity
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HIV-1 / physiology
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Humans
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In Vitro Techniques
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Protein Interaction Domains and Motifs
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Protein Subunits
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transfection
Substances
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Ether-A-Go-Go Potassium Channels
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HIV Envelope Protein gp120
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KCNH3 protein, human
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Nerve Tissue Proteins
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Protein Subunits
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RNA, Messenger
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Recombinant Fusion Proteins
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gp120 protein, Human immunodeficiency virus 1