Abstract
The primary structures of DNA-polymerase (ul30) and thymidine kinase (ul23) genes from several herpes simplex virus type 1 (HSV-1) clinical isolates which differed in sensitivity for a number of antiherpetic drugs were determined and compared with those for two laboratory HSV-1 strains one of which was ACV-sensitive (L2), while the another was resistant (L2) to ACV. The phylogenetic analysis of the sequences showed that conserved regions of ul30 gene of HSV-1 clinical isolates and L2 strain were homologous with the exception of point mutations and degenerated substitutions. Several new mutations in the HSV-1 DNA-polymerase and thymidine kinase functional domains were established and identified as the substitutions associated with the strain-resistance to ACV and other drugs.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Acyclovir / pharmacology*
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Antiviral Agents / pharmacology*
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Cell Line
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DNA-Directed DNA Polymerase / genetics*
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DNA-Directed DNA Polymerase / metabolism
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Drug Resistance, Viral / genetics*
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Exodeoxyribonucleases / genetics*
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Exodeoxyribonucleases / metabolism
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Herpes Simplex / drug therapy
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Herpes Simplex / enzymology
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Herpes Simplex / genetics*
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Herpesvirus 1, Human / enzymology
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Herpesvirus 1, Human / genetics*
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Herpesvirus 1, Human / isolation & purification
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Humans
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Point Mutation*
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Thymidine Kinase / genetics*
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Thymidine Kinase / metabolism
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Viral Proteins / genetics*
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Viral Proteins / metabolism
Substances
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Antiviral Agents
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Viral Proteins
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Thymidine Kinase
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DNA-Directed DNA Polymerase
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Exodeoxyribonucleases
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DNA polymerase, Simplexvirus
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Acyclovir