Abstract
The Ada-Two-A-containing (ATAC) histone acetyltransferase and Mediator coactivator complexes regulate independent and distinct steps during transcription initiation and elongation. Here, we report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. Moreover, we identify leucine zipper motif-containing protein 1 as a subunit of this meta-coactivator complex (MECO). Finally, we demonstrate that the MECO regulates a subset of RNA polymerase II-transcribed non-coding RNA genes. Our findings establish that transcription coactivator complexes can form stable subcomplexes to facilitate their combined actions on specific target genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Chemokines, C / chemistry
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Chemokines, C / genetics
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Chemokines, C / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Fibroblasts / cytology
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Fibroblasts / physiology
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Gene Expression Regulation
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Leucine Zippers
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Mass Spectrometry
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Mediator Complex / chemistry
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Mediator Complex / genetics
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Mediator Complex / metabolism*
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Mice
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Mice, Knockout
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Multiprotein Complexes / metabolism*
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Peptides / genetics
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Peptides / metabolism
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Protein Subunits / chemistry
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Protein Subunits / genetics
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Protein Subunits / metabolism
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RNA, Untranslated / genetics*
Substances
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Chemokines, C
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DNA-Binding Proteins
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Luzp1 protein, mouse
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Mediator Complex
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Multiprotein Complexes
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Peptides
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Protein Subunits
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RNA, Untranslated
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Xcl1 protein, mouse