Accumulation of caveolin-3 protein is limited in damaged muscle in chicken muscular dystrophy

Comp Biochem Physiol A Mol Integr Physiol. 2010 Sep;157(1):68-72. doi: 10.1016/j.cbpa.2010.04.019. Epub 2010 May 6.

Abstract

Members of the caveolin family are the main component of caveolae, and caveolin-3 is a muscle-specific protein. Caveolin-3 deficiency induces a muscular dystrophic phenotype, while its overexpression is also harmful to muscle cells. Increased caveolae were observed in chicken muscular dystrophy; however, the underlying mechanism causing the onset remains unclear. Therefore, the current study analyzes the expression of caveolin-3 and other caveola-related proteins in dystrophic chickens. Western blotting and semi-quantitative RT-PCR analysis revealed that (1) caveolin-3 is highly expressed in the damaged muscle of dystrophic chickens (7.12-fold); (2) the amount of caveolin-3 protein is regulated in posttranslational modification, since no significant increase is observed at the mRNA level (1.09-fold); and (3) the expression pattern of other caveola-related proteins is similar to that of caveolin-3. These results suggest that the accumulation of caveolin-3 protein may be associated with the causative process of chicken muscular dystrophy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caveolin 3 / genetics*
  • Caveolin 3 / metabolism*
  • Chickens
  • Female
  • Male
  • Muscle, Skeletal / metabolism*
  • Muscle, Skeletal / pathology*
  • Muscular Dystrophies / genetics*
  • Muscular Dystrophies / metabolism*
  • Muscular Dystrophies / pathology
  • Phenotype
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Caveolin 3