A retroviral vector common integration site between leupaxin and zinc finger protein 91 (ZFP91) observed in baboon hematopoietic repopulating cells

Exp Hematol. 2010 Sep;38(9):819-22, 822.e1-3. doi: 10.1016/j.exphem.2010.04.014. Epub 2010 Apr 29.

Abstract

Objective: Retroviral vector proviruses can lead to aberrant expression of nearby genes in hematopoietic repopulating cells, leading to an over-representation of clones with dysregulated genes that affect hematopoiesis. Common integration sites (CISs) identified using the vector provirus as a molecular tag can be used to identify these genes. Here we characterized a retroviral CIS observed at high frequency in baboon hematopoietic repopulating cells that has not been described previously.

Materials and methods: Gammaretroviral vector integration sites in baboon repopulating cells identified by polymerase chain reaction amplification were localized to the human genome to identify a CIS. The presence of each clone was tracked over time using allele-specific polymerase chain reaction.

Results: In three different animals that received gammaretrovirally transduced CD34-enriched bone marrow cells, vector proviruses were identified at three distinct sites within a window of 664 base pairs between leupaxin and zinc finger protein 91 (ZFP91). All three integrants of the CIS occurred within a CpG island between leupaxin and zinc finger protein 91 (ZFP91).

Conclusions: We describe a novel CIS between leupaxin and ZFP91 in hematopoietic repopulating cells. Our data suggest that leupaxin and/or ZFP91 may play a role in hematopoietic repopulating cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antigens, CD34
  • Bone Marrow Transplantation
  • Gammaretrovirus / genetics*
  • Genetic Vectors / genetics*
  • Hematopoietic Stem Cells*
  • Humans
  • Papio
  • Phosphoproteins / genetics*
  • Proviruses / genetics*
  • Repressor Proteins / genetics*
  • Transduction, Genetic / methods
  • Virus Integration / genetics*
  • Zinc Fingers*

Substances

  • Antigens, CD34
  • Phosphoproteins
  • Repressor Proteins