Abstract
A family of transcriptional activators has recently been identified in chickens; these transcriptional activators recognize a common consensus motif (WGATAR) through a conserved C4 zinc finger DNA-binding domain. One of the members of this multigene family, cGATA-3, is most abundantly expressed in the T-lymphocyte cell lineage. Analysis of human and murine GATA-3 factors shows a striking degree of amino acid sequence identity and similar patterns of tissue specificity of expression in these three organisms. The murine and human factors are abundantly expressed in a variety of human and murine T-cell lines and can activate transcription through a tissue-specific GATA-binding site identified within the human T-cell receptor delta gene enhancer. We infer that the murine and human GATA-3 proteins play a central and highly conserved role in vertebrate T-cell-specific transcriptional regulation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Chickens
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Enhancer Elements, Genetic*
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GATA3 Transcription Factor
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Gene Library
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Globins / genetics
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Humans
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Mice
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Molecular Sequence Data
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Multigene Family*
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Oligonucleotide Probes
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Rabbits
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Receptors, Antigen, T-Cell / genetics*
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Regulatory Sequences, Nucleic Acid*
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Sequence Homology, Nucleic Acid
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T-Lymphocytes / immunology*
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TATA Box
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Trans-Activators / genetics*
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Trans-Activators / metabolism
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Transcription Factors / genetics*
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Transcription, Genetic*
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Transfection
Substances
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DNA-Binding Proteins
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GATA3 Transcription Factor
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GATA3 protein, human
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Gata3 protein, mouse
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Oligonucleotide Probes
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Receptors, Antigen, T-Cell
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Trans-Activators
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Transcription Factors
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Globins
Associated data
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GENBANK/M64822
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GENBANK/M64823
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GENBANK/M64824
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GENBANK/M64825
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GENBANK/M64826
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GENBANK/M64827
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GENBANK/X55122
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GENBANK/X55123
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GENBANK/X56930
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GENBANK/X56931