Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer

Mol Cell Biol. 1991 May;11(5):2778-84. doi: 10.1128/mcb.11.5.2778-2784.1991.

Abstract

A family of transcriptional activators has recently been identified in chickens; these transcriptional activators recognize a common consensus motif (WGATAR) through a conserved C4 zinc finger DNA-binding domain. One of the members of this multigene family, cGATA-3, is most abundantly expressed in the T-lymphocyte cell lineage. Analysis of human and murine GATA-3 factors shows a striking degree of amino acid sequence identity and similar patterns of tissue specificity of expression in these three organisms. The murine and human factors are abundantly expressed in a variety of human and murine T-cell lines and can activate transcription through a tissue-specific GATA-binding site identified within the human T-cell receptor delta gene enhancer. We infer that the murine and human GATA-3 proteins play a central and highly conserved role in vertebrate T-cell-specific transcriptional regulation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chickens
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Enhancer Elements, Genetic*
  • GATA3 Transcription Factor
  • Gene Library
  • Globins / genetics
  • Humans
  • Mice
  • Molecular Sequence Data
  • Multigene Family*
  • Oligonucleotide Probes
  • Rabbits
  • Receptors, Antigen, T-Cell / genetics*
  • Regulatory Sequences, Nucleic Acid*
  • Sequence Homology, Nucleic Acid
  • T-Lymphocytes / immunology*
  • TATA Box
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transcription Factors / genetics*
  • Transcription, Genetic*
  • Transfection

Substances

  • DNA-Binding Proteins
  • GATA3 Transcription Factor
  • GATA3 protein, human
  • Gata3 protein, mouse
  • Oligonucleotide Probes
  • Receptors, Antigen, T-Cell
  • Trans-Activators
  • Transcription Factors
  • Globins

Associated data

  • GENBANK/M64822
  • GENBANK/M64823
  • GENBANK/M64824
  • GENBANK/M64825
  • GENBANK/M64826
  • GENBANK/M64827
  • GENBANK/X55122
  • GENBANK/X55123
  • GENBANK/X56930
  • GENBANK/X56931