Myeloid translocation gene 16b is a dual A-kinase anchoring protein that interacts selectively with plexins in a phospho-regulated manner

FEBS Lett. 2010 Mar 5;584(5):873-7. doi: 10.1016/j.febslet.2010.02.007. Epub 2010 Feb 9.

Abstract

The myeloid translocation gene (MTG) homologue Nervy associates with PlexinA on the plasma membrane, where it functions as an A-kinase anchoring protein (AKAP) to modulate plexin-mediated semaphorin signaling in Drosophila. Mammalian MTG16b is an AKAP found in immune cells where plexin-mediated semaphorin signaling regulates immune responses. This study provides the first evidence that MTG16b is a dual AKAP capable of binding plexins. These interactions are selective (PlexinA1 and A3 bind MTG, while PlexinB1 does not) and can be regulated by PKA-phosphorylation. Collectively, these data suggest a possible mechanism for the targeting and integration of adenosine 3',5'-cyclic monophosphate (cAMP) and semaphorin signaling in immune cells.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • A Kinase Anchor Proteins / genetics
  • A Kinase Anchor Proteins / metabolism*
  • Animals
  • COS Cells
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism*
  • Chlorocebus aethiops
  • Cyclic AMP-Dependent Protein Kinase Type I / genetics
  • Cyclic AMP-Dependent Protein Kinase Type I / metabolism
  • Cyclic AMP-Dependent Protein Kinase Type II / genetics
  • Cyclic AMP-Dependent Protein Kinase Type II / metabolism
  • Humans
  • Immunoblotting
  • Immunoprecipitation
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Mice
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Protein Binding
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Tumor Suppressor Proteins / genetics
  • Tumor Suppressor Proteins / metabolism*

Substances

  • A Kinase Anchor Proteins
  • CBFA2T3 protein, human
  • Cell Adhesion Molecules
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • PLXNA1 protein, human
  • PLXNA3 protein, human
  • PLXNB1 protein, human
  • Phosphoproteins
  • Plxna1 protein, mouse
  • Receptors, Cell Surface
  • Repressor Proteins
  • Tumor Suppressor Proteins
  • plexin
  • Cyclic AMP-Dependent Protein Kinase Type I
  • Cyclic AMP-Dependent Protein Kinase Type II