Abstract
The myeloid translocation gene (MTG) homologue Nervy associates with PlexinA on the plasma membrane, where it functions as an A-kinase anchoring protein (AKAP) to modulate plexin-mediated semaphorin signaling in Drosophila. Mammalian MTG16b is an AKAP found in immune cells where plexin-mediated semaphorin signaling regulates immune responses. This study provides the first evidence that MTG16b is a dual AKAP capable of binding plexins. These interactions are selective (PlexinA1 and A3 bind MTG, while PlexinB1 does not) and can be regulated by PKA-phosphorylation. Collectively, these data suggest a possible mechanism for the targeting and integration of adenosine 3',5'-cyclic monophosphate (cAMP) and semaphorin signaling in immune cells.
Published by Elsevier B.V.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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A Kinase Anchor Proteins / genetics
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A Kinase Anchor Proteins / metabolism*
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Animals
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COS Cells
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / metabolism*
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Chlorocebus aethiops
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Cyclic AMP-Dependent Protein Kinase Type I / genetics
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Cyclic AMP-Dependent Protein Kinase Type I / metabolism
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Cyclic AMP-Dependent Protein Kinase Type II / genetics
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Cyclic AMP-Dependent Protein Kinase Type II / metabolism
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Humans
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Immunoblotting
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Immunoprecipitation
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism
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Mice
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Phosphorylation
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Protein Binding
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Tumor Suppressor Proteins / genetics
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Tumor Suppressor Proteins / metabolism*
Substances
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A Kinase Anchor Proteins
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CBFA2T3 protein, human
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Cell Adhesion Molecules
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Membrane Glycoproteins
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Nerve Tissue Proteins
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PLXNA1 protein, human
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PLXNA3 protein, human
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PLXNB1 protein, human
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Phosphoproteins
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Plxna1 protein, mouse
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Receptors, Cell Surface
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Repressor Proteins
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Tumor Suppressor Proteins
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plexin
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Cyclic AMP-Dependent Protein Kinase Type I
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Cyclic AMP-Dependent Protein Kinase Type II