Organization and chromosomal localization of the human platelet-derived endothelial cell growth factor gene

Mol Cell Biol. 1991 Apr;11(4):2125-32. doi: 10.1128/mcb.11.4.2125-2132.1991.

Abstract

Human platelet-derived endothelial cell growth factor (hPD-ECGF) is a novel angiogenic factor which stimulates endothelial cell growth in vitro and promotes angiogenesis in vivo. We report here the cloning and sequencing of the gene for hPD-ECGF and its flanking regions. This gene is composed of 10 exons dispersed over a 4.3-kb region. Its promoter lacks a TATA box and a CCAAT box, structures characteristic of eukaryotic promoters. Instead, six copies of potential Sp1-binding sites (GGGCGG or CCGCCC) were clustered just upstream of the transcription start sites. Southern blot analysis using genomic DNAs from several vertebrates suggested that the gene for PD-ECGF is conserved phylogenetically among vertebrates. The gene for hPD-ECGF was localized to chromosome 22 by analysis of a panel of human-rodent somatic cell hybrid lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Chromosomes, Human, Pair 22*
  • Cloning, Molecular
  • Endothelium, Vascular / cytology*
  • Exons
  • Female
  • Genes
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • Placenta / metabolism
  • Pregnancy
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Thymidine Phosphorylase / genetics*
  • Transcription, Genetic

Substances

  • Thymidine Phosphorylase

Associated data

  • GENBANK/M58602
  • GENBANK/M64819
  • GENBANK/M64820
  • GENBANK/M64821
  • GENBANK/M64822
  • GENBANK/M64823
  • GENBANK/M64824
  • GENBANK/M64825
  • GENBANK/M64826
  • GENBANK/M64827