Abstract
The metastasis of malignant tumor cells from the primary tumor to distant sites in the body is a complex process. To identify genes that may be essential for metastasis, we established poorly metastatic mouse melanoma cells, namely Y925F-mutated FAK-transfected cells (Y925F cells), from the highly metastatic mouse melanoma cell line B16F10, and performed expression analyses. The expression of phospholipid protein 2 (PLP2) was markedly down-regulated in the Y925F cells. To elucidate the function of PLP2, we established melanoma cells overexpressing PLP2. We found that PLP2 enhanced proliferation, adhesion, invasion, and MMP-2 secretion in vitro, and tumor metastasis in vivo. These results suggest that PLP2 aids metastasis. Furthermore, we showed that PLP2 binds specifically to PI3K, thus activating Akt.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Adhesion / genetics
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Cell Movement / genetics
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Enzyme Activation
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Gene Expression Regulation, Neoplastic
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Lung Neoplasms / genetics
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Lung Neoplasms / secondary
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MARVEL Domain-Containing Proteins
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Matrix Metalloproteinase 2 / metabolism
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Melanoma, Experimental / genetics*
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Melanoma, Experimental / pathology*
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Membrane Proteins
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Mice
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Mice, Inbred C57BL
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Myelin Proteolipid Protein / genetics
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Myelin Proteolipid Protein / metabolism
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Myelin Proteolipid Protein / physiology*
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Neoplasm Metastasis
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Oncogene Protein v-akt / metabolism
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Phosphatidylinositol 3-Kinases / metabolism
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Proteolipids
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Rabbits
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Skin Neoplasms / genetics*
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Skin Neoplasms / pathology*
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Tumor Cells, Cultured
Substances
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MARVEL Domain-Containing Proteins
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Membrane Proteins
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Myelin Proteolipid Protein
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Plp2 protein, mouse
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Proteolipids
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Phosphatidylinositol 3-Kinases
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Oncogene Protein v-akt
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Matrix Metalloproteinase 2