Mouse ZAR1-like (XM_359149) colocalizes with mRNA processing components and its dominant-negative mutant caused two-cell-stage embryonic arrest

Jianjun Hu; Fengchao Wang; Xiaoquan Zhu; Ye Yuan; Mingxiao Ding; Shaorong Gao

doi:10.1002/dvdy.22170

Mouse ZAR1-like (XM_359149) colocalizes with mRNA processing components and its dominant-negative mutant caused two-cell-stage embryonic arrest

Dev Dyn. 2010 Feb;239(2):407-24. doi: 10.1002/dvdy.22170.

Authors

Jianjun Hu¹, Fengchao Wang, Xiaoquan Zhu, Ye Yuan, Mingxiao Ding, Shaorong Gao

Affiliation

¹ The Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, People's Republic of China.

PMID: 20014101
DOI: 10.1002/dvdy.22170

Abstract

Maternal effect genes and encoding proteins are necessary for nuclear reprogramming and zygotic genome activation. However, the mechanisms that mediate these functions are largely unknown. Here we identified XM_359149, a Zar1-like gene that is predominantly expressed in oocytes and zygotes, which we designated Zar1-like (Zar1l). ZAR1L-EGFP formed multiple cytoplasmic foci in late two-cell-stage embryos. Expression of the ZAR1L C-terminus induced two-cell-stage embryonic arrest, accompanied with abnormal methylation of histone H3K4me2/3 and H3K9me2/3, and marked down-regulation of a group of chromatin modification factors including Dppa2, Dppa4, and Piwil2. When ectopically expressed in somatic cells, ZAR1L colocalized with P-body components including EIF2C1(AGO1), EIF2C2(AGO2), DDX6 and LSM14A, and germline-specific chromatoid body components including PIWIL1, PIWIL2, and LIN28. ZAR1L colocalized with ZAR1 and interacted with human LIN28. Our data suggest that ZAR1L and ZAR1 may comprise a novel family of processing-body/chromatoid-body components that potentially function as RNA regulators in early embryos.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Argonaute Proteins
Bromodeoxyuridine
Cell Line
DEAD-box RNA Helicases / metabolism
Egg Proteins / metabolism
Embryo, Mammalian / metabolism*
Embryonic Development*
Eukaryotic Initiation Factor-2 / metabolism
Eukaryotic Initiation Factors / metabolism
Female
Gene Expression Regulation, Developmental
Histones / metabolism
Humans
Male
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Molecular Sequence Data
Mutation
Proteins / genetics
Proteins / metabolism*
Proto-Oncogene Proteins / metabolism
RNA Polymerase II / metabolism
RNA, Messenger / metabolism
RNA-Binding Proteins / metabolism
Sequence Alignment
Transcription Factors

Substances

Ago1 protein, mouse
Ago2 protein, mouse
Argonaute Proteins
Egg Proteins
Eukaryotic Initiation Factor-2
Eukaryotic Initiation Factors
Histones
LSM14A protein, mouse
Lin-28 protein, mouse
Lin28A protein, human
Piwil1 protein, mouse
Piwil2 protein, mouse
Proteins
Proto-Oncogene Proteins
RNA, Messenger
RNA-Binding Proteins
Transcription Factors
ZAR1L protein, mouse
Zar1 protein, mouse
RNA Polymerase II
Ddx6 protein, mouse
DEAD-box RNA Helicases
Bromodeoxyuridine