Generation of a zinc finger protein ZPR1 mutant that constitutively interacted with translation elongation factor 1alpha

Noriyuki Yanaka; Yurika Kaseda; Anna Tanaka; Yoshihito Nogusa; Naoki Sumiyoshi; Norihisa Kato

doi:10.1271/bbb.90745

Generation of a zinc finger protein ZPR1 mutant that constitutively interacted with translation elongation factor 1alpha

Biosci Biotechnol Biochem. 2009 Dec;73(12):2809-11. doi: 10.1271/bbb.90745. Epub 2009 Dec 7.

Authors

Noriyuki Yanaka¹, Yurika Kaseda, Anna Tanaka, Yoshihito Nogusa, Naoki Sumiyoshi, Norihisa Kato

Affiliation

¹ Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan. yanaka@hiroshima-u.ac.jp

PMID: 19966453
DOI: 10.1271/bbb.90745

Abstract

Zinc finger protein ZPR1 (ZPR1) binds to eukaryotic translation elongation factor 1alpha (eEF1alpha) in response to growth stimuli, and is also involved in transcription and cell cycle regulation. In this study, we characterized the interaction of ZPR1 and eEF1alpha and generated a ZPR1 mutant that constitutively interacted with eEF1alpha. ZPR1-DeltaA (Delta193-246) bound to eEF1alpha independently of Zn(2+) in vivo. This study indicates that ZPR1-DeltaA (Delta193-246) is a useful tool to provide structural insights into ZPR1 and to investigate the biological significance of the interaction between ZPR1 and eEF1alpha.

MeSH terms

Animals
Carrier Proteins / genetics*
Carrier Proteins / metabolism*
Cell Line
Humans
Intracellular Signaling Peptides and Proteins
Mice
Mutant Proteins / genetics*
Mutant Proteins / metabolism*
Mutation*
Peptide Elongation Factor 1 / metabolism*
Protein Binding

Substances

Carrier Proteins
Intracellular Signaling Peptides and Proteins
Mutant Proteins
Peptide Elongation Factor 1
Zfp259 protein, mouse