Rab36 regulates the spatial distribution of late endosomes and lysosomes through a similar mechanism to Rab34

Mol Membr Biol. 2010 Jan;27(1):23-30. doi: 10.3109/09687680903417470.

Abstract

Small GTPase Rab36 is homologous to Rab34 with 56% amino acid sequence identity. Rab34 was characterized as a Golgi-associated Rab protein and regulates lysosomal positioning through interaction with RILP; however, the properties and functions of Rab36 have not been investigated. To investigate Rab36, we constructed EGFP-Rab36 wild type, the active GTP-bound mutant EGFP-Rab36Q116L and negative GDP-bound mutant EGFP-Rab36T71N. Expression of EGFP-Rab36 wild type revealed that Rab36 co-localized with Golgi markers GM130, Syntaxin 5 and TGN46 in Hela cells, indicating Rab36 is associated with Golgi apparatus. Over-expression of Rab36 induced late endosome and lysosome clustering around the Golgi apparatus, marked by LBPA, CD63, Lamp1 and Lamp2, without effects on early endosomal compartment marked by EEA1. GST-pulldown assay demonstrated that Rab36 can also interact with RILP. In addition, the binding region for Rab36 is in the C-terminal region (aa199-401) of RILP. Our data suggested that Rab36 may regulate the spatial distribution of late endosomes and lysosomes through a similar mechanism to Rab34.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism
  • Amino Acid Substitution
  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • Autoantigens / genetics
  • Autoantigens / metabolism
  • Endosomes / genetics
  • Endosomes / metabolism*
  • Golgi Apparatus / genetics
  • Golgi Apparatus / metabolism*
  • HeLa Cells
  • Humans
  • Lysosomal Membrane Proteins / genetics
  • Lysosomal Membrane Proteins / metabolism
  • Lysosomal-Associated Membrane Protein 2
  • Lysosomes / genetics
  • Lysosomes / metabolism*
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Mutation, Missense
  • Platelet Membrane Glycoproteins / genetics
  • Platelet Membrane Glycoproteins / metabolism
  • Qa-SNARE Proteins / genetics
  • Qa-SNARE Proteins / metabolism
  • Tetraspanin 30
  • rab GTP-Binding Proteins / genetics
  • rab GTP-Binding Proteins / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • Antigens, CD
  • Autoantigens
  • CD63 protein, human
  • Golgin subfamily A member 2
  • LAMP1 protein, human
  • LAMP2 protein, human
  • Lysosomal-Associated Membrane Protein 2
  • Lysosomal Membrane Proteins
  • Membrane Glycoproteins
  • Membrane Proteins
  • Platelet Membrane Glycoproteins
  • Qa-SNARE Proteins
  • RILP protein, human
  • TGOLN2 protein, human
  • Tetraspanin 30
  • RAB39A protein, human
  • Rab36 protein, human
  • rab GTP-Binding Proteins