Abstract
Using isogenic clinical bloodstream Staphylococcus aureus strains from a patient with relapsing endocarditis, we investigated the transcriptional profiles of the mprF and dlt genes in the context of cell-surface charge and daptomycin nonsusceptibility. As in prior studies, a point mutation within mprF was observed in the daptomycin-nonsusceptible strain. However, neither the transcriptional profile of mprF nor the membrane phospholipid analyses were compatible with the anticipated mprF gain-in-function phenotype. In contrast, we demonstrated enhanced dlt expression coincident with increased positive surface charge and reduced daptomycin binding.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / metabolism
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / genetics
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Colony Count, Microbial
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Daptomycin / metabolism
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Daptomycin / pharmacology*
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Drug Resistance, Bacterial*
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Endocarditis, Bacterial / microbiology*
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Gene Expression
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Gene Expression Profiling
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Genes, Bacterial
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Humans
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Point Mutation
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Staphylococcal Infections / microbiology*
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / isolation & purification
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Staphylococcus aureus / physiology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Daptomycin