Early expression of nucleolar SURF-6 protein in mouse spleen lymphocytes activated for proliferation in vitro

Bull Exp Biol Med. 2009 May;147(5):578-82. doi: 10.1007/s10517-009-0578-z.
[Article in English, Russian]

Abstract

Using specific antibodies we studied the content of nucleolar SURF-6 protein, which participates in rRNA processing, in mouser spleen lymphocytes activated for proliferation with concanavalin A and compared it with the content of nucleolar nucleophosmin/B23 protein and DNA replication factor PCNA, well-known markers of proliferating cells. Using immunocytochemistry and immunoblotting methods we demonstrate that the concentration of all these proteins increases simultaneously with increasing the proportion of proliferating cells. Unlike nucleophosmin/B23, SURF-6 protein was not revealed in quiescent lymphocyte nucleoli, while the increase of its level in activated lymphocytes preceded elevation of PCNA level. These observations suggest that nucleolar protein SURF-6 can act as a marker of early T lymphocyte activation for proliferation and that it could participate in cell cycle regulation in mammals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Concanavalin A / pharmacology
  • Flow Cytometry
  • Gene Expression Regulation*
  • Immunoblotting
  • Immunohistochemistry
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / physiology
  • Lymphocytes / cytology*
  • Lymphocytes / drug effects
  • Lymphocytes / metabolism*
  • Mice
  • Mitogens / pharmacology
  • Nuclear Proteins / metabolism*
  • Nucleophosmin
  • Proliferating Cell Nuclear Antigen / metabolism
  • Spleen / cytology*

Substances

  • Mitogens
  • Npm1 protein, mouse
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen
  • Surf6 protein, mouse
  • Concanavalin A
  • Nucleophosmin