Early expression of nucleolar SURF-6 protein in mouse spleen lymphocytes activated for proliferation in vitro
Bull Exp Biol Med. 2009 May;147(5):578-82.
doi: 10.1007/s10517-009-0578-z.
[Article in
English,
Russian]
Affiliation
- 1 M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. a_moraleva@mail.ru
Abstract
Using specific antibodies we studied the content of nucleolar SURF-6 protein, which participates in rRNA processing, in mouser spleen lymphocytes activated for proliferation with concanavalin A and compared it with the content of nucleolar nucleophosmin/B23 protein and DNA replication factor PCNA, well-known markers of proliferating cells. Using immunocytochemistry and immunoblotting methods we demonstrate that the concentration of all these proteins increases simultaneously with increasing the proportion of proliferating cells. Unlike nucleophosmin/B23, SURF-6 protein was not revealed in quiescent lymphocyte nucleoli, while the increase of its level in activated lymphocytes preceded elevation of PCNA level. These observations suggest that nucleolar protein SURF-6 can act as a marker of early T lymphocyte activation for proliferation and that it could participate in cell cycle regulation in mammals.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cells, Cultured
-
Concanavalin A / pharmacology
-
Flow Cytometry
-
Gene Expression Regulation*
-
Immunoblotting
-
Immunohistochemistry
-
Lymphocyte Activation / drug effects
-
Lymphocyte Activation / physiology
-
Lymphocytes / cytology*
-
Lymphocytes / drug effects
-
Lymphocytes / metabolism*
-
Mice
-
Mitogens / pharmacology
-
Nuclear Proteins / metabolism*
-
Nucleophosmin
-
Proliferating Cell Nuclear Antigen / metabolism
-
Spleen / cytology*
Substances
-
Mitogens
-
Npm1 protein, mouse
-
Nuclear Proteins
-
Proliferating Cell Nuclear Antigen
-
Surf6 protein, mouse
-
Concanavalin A
-
Nucleophosmin