A novel neural-specific BMP antagonist, Brorin-like, of the Chordin family

Hiroyuki Miwa; Ayumi Miyake; Yuya Kouta; Akihiko Shimada; Yuki Yamashita; Yoshiaki Nakayama; Hajime Yamauchi; Morichika Konishi; Nobuyuki Itoh

doi:10.1016/j.febslet.2009.10.044

A novel neural-specific BMP antagonist, Brorin-like, of the Chordin family

FEBS Lett. 2009 Nov 19;583(22):3643-8. doi: 10.1016/j.febslet.2009.10.044. Epub 2009 Oct 21.

Authors

Hiroyuki Miwa¹, Ayumi Miyake, Yuya Kouta, Akihiko Shimada, Yuki Yamashita, Yoshiaki Nakayama, Hajime Yamauchi, Morichika Konishi, Nobuyuki Itoh

Affiliation

¹ Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Yoshida-shimoadachi, Sakyo, Kyoto 606-8501, Japan.

PMID: 19852960
DOI: 10.1016/j.febslet.2009.10.044

Abstract

We identified a gene encoding a novel secreted protein in mice, humans, and zebrafish. As the protein of 222 amino acids is similar to Brorin, a secreted BMP antagonist, which is a member of the Chordin family, we named it Brorin-like. Recombinant Brorin-like protein weakly but significantly inhibited the activity of BMP in mouse preosteoblastic cells and promoted neurogenesis in mouse neural precursor cells. Brorin-like was predominantly expressed in the adult brain and embryonic neural tissues. The inhibition of Brorin-like functions in zebrafish resulted in the impairment of neural development. Brorin-like potentially plays roles in neural development and functions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Amino Acid Sequence
Animals
Astrocytes / cytology
Astrocytes / metabolism
Bone Morphogenetic Proteins / antagonists & inhibitors
Bone Morphogenetic Proteins / metabolism*
COS Cells
Cell Differentiation / genetics
Cells, Cultured
Chlorocebus aethiops
Gene Expression Profiling
Gene Expression Regulation, Developmental
Glycoproteins / genetics*
Glycoproteins / metabolism
Glycoproteins / pharmacology
Humans
In Situ Hybridization
Intercellular Signaling Peptides and Proteins / genetics*
Intercellular Signaling Peptides and Proteins / metabolism
Intercellular Signaling Peptides and Proteins / pharmacology
Mice
Molecular Sequence Data
Nerve Tissue Proteins / genetics*
Neurons / cytology
Neurons / metabolism*
Recombinant Proteins / metabolism
Recombinant Proteins / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Signal Transduction / drug effects
Zebrafish / embryology
Zebrafish / genetics
Zebrafish Proteins / genetics*
Zebrafish Proteins / metabolism

Substances

Bone Morphogenetic Proteins
Glycoproteins
Intercellular Signaling Peptides and Proteins
Nerve Tissue Proteins
Recombinant Proteins
VWC2L protein, human
Vwc2l protein, mouse
Zebrafish Proteins
vwc2l protein, zebrafish
chordin