Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus

A I Glukhov; S A Gordeev; S V Vinogradov; V I Kiselev; V M Kramarov; O I Kiselev; E S Severin

doi:10.1016/0890-8508(90)90002-h

Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus

Mol Cell Probes. 1990 Dec;4(6):435-43. doi: 10.1016/0890-8508(90)90002-h.

Authors

A I Glukhov¹, S A Gordeev, S V Vinogradov, V I Kiselev, V M Kramarov, O I Kiselev, E S Severin

Affiliation

¹ Research Center of Molecular Diagnostics, USSR Ministry of Health, Moscow.

PMID: 1965009
DOI: 10.1016/0890-8508(90)90002-h

Abstract

Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.

MeSH terms

Base Sequence
DNA Probes
DNA, Viral / genetics*
DNA-Directed DNA Polymerase / metabolism*
Electrophoresis, Polyacrylamide Gel
HIV / genetics*
Herpesvirus 4, Human / genetics*
Molecular Sequence Data
Polymerase Chain Reaction*
Thermus / enzymology*

Substances

DNA Probes
DNA, Viral
DNA-Directed DNA Polymerase