Cyclin Y, a novel membrane-associated cyclin, interacts with PFTK1

Mei Jiang; Yankun Gao; Tao Yang; Xueliang Zhu; Jiangye Chen

doi:10.1016/j.febslet.2009.06.010

Cyclin Y, a novel membrane-associated cyclin, interacts with PFTK1

FEBS Lett. 2009 Jul 7;583(13):2171-8. doi: 10.1016/j.febslet.2009.06.010. Epub 2009 Jun 12.

Authors

Mei Jiang¹, Yankun Gao, Tao Yang, Xueliang Zhu, Jiangye Chen

Affiliation

¹ State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, SIBS, Chinese Academy of Sciences, Shanghai, China.

PMID: 19524571
DOI: 10.1016/j.febslet.2009.06.010

Abstract

A novel cyclin, CCNY, was identified as a PFTK1 interacting protein in a yeast two-hybrid screen. The cyclin box in CCNY and the PFTAIRE motif in PFTK1 are both required for the interaction which was confirmed by in vivo and in vitro assays. Two transcripts (4 and 2kb), of CCNY were detected by Northern blot analysis and CCNY was enriched at the plasma membrane due to an N-terminal myristoylation signal. We propose that binding of CCNY to PFTK1 enhances PFTK1 kinase activity and changes its intracellular location.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
CDC2 Protein Kinase / chemistry
CDC2 Protein Kinase / metabolism*
Cell Membrane / metabolism
Cyclin-Dependent Kinases
Cyclins / chemistry
Cyclins / metabolism*
Humans
Membrane Proteins / chemistry
Membrane Proteins / metabolism*
Microscopy, Fluorescence
Molecular Sequence Data
Transfection
Two-Hybrid System Techniques

Substances

CCNY protein, human
Cyclins
Membrane Proteins
CDC2 Protein Kinase
CDK14 protein, human
Cyclin-Dependent Kinases