TIPT2 and geminin interact with basal transcription factors to synergize in transcriptional regulation

Mara E Pitulescu; Martin Teichmann; Lingfei Luo; Michael Kessel

doi:10.1186/1471-2091-10-16

TIPT2 and geminin interact with basal transcription factors to synergize in transcriptional regulation

BMC Biochem. 2009 Jun 10:10:16. doi: 10.1186/1471-2091-10-16.

Authors

Mara E Pitulescu¹, Martin Teichmann, Lingfei Luo, Michael Kessel

Affiliation

¹ Department of Molecular Cell Biology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany. mpitule@gwdg.de

Abstract

Background: The re-replication inhibitor Geminin binds to several transcription factors including homeodomain proteins, and to members of the polycomb and the SWI/SNF complexes.

Results: Here we describe the TATA-binding protein-like factor-interacting protein (TIPT) isoform 2, as a strong binding partner of Geminin. TIPT2 is widely expressed in mouse embryonic and adult tissues, residing both in cyto- and nucleoplasma, and enriched in the nucleolus. Like Geminin, also TIPT2 interacts with several polycomb factors, with the general transcription factor TBP (TATA box binding protein), and with the related protein TBPL1 (TRF2). TIPT2 synergizes with geminin and TBP in the activation of TATA box-containing promoters, and with TBPL1 and geminin in the activation of the TATA-less NF1 promoter. Geminin and TIPT2 were detected in the chromatin near TBP/TBPL1 binding sites.

Conclusion: Together, our study introduces a novel transcriptional regulator and its function in cooperation with chromatin associated factors and the basal transcription machinery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Cell Cycle Proteins / chemistry
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
Cell Line
Cell Nucleus / genetics
Cell Nucleus / metabolism
Chromatin / metabolism
Chromosomal Proteins, Non-Histone / chemistry
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism*
Geminin
Gene Expression Regulation*
HeLa Cells
Humans
Mice
Molecular Sequence Data
Nuclear Proteins / chemistry
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Promoter Regions, Genetic
Protein Binding
Protein Transport
TATA-Box Binding Protein / genetics
TATA-Box Binding Protein / metabolism
Transcription Factors / chemistry
Transcription Factors / genetics
Transcription Factors / metabolism*
Transcription, Genetic*
Transcriptional Activation

Substances

Cell Cycle Proteins
Chromatin
Chromosomal Proteins, Non-Histone
Geminin
Gmnn protein, mouse
Nuclear Proteins
TATA-Box Binding Protein
TIPT protein, mouse
Transcription Factors