Effects of 3,4-dichloroaniline on testicle enzymes as biological markers in rats

Biomed Environ Sci. 2009 Feb;22(1):40-3. doi: 10.1016/S0895-3988(09)60020-9.

Abstract

Objective: To investigate the effects of 3,4-dichloroaniline (3,4-DCA) on activities of testicle enzymes as biological markers in rats.

Methods: Fifty male rats were randomly divided into 5 groups (n=10). One group was left untreated and used as a solvent control (administered orally by corn oil), while the other 4 groups were treated with 3, 4-DCA. Corn oil was used as a solvent, and 3,4-DCA was diluted into tested concentrations (39, 81, 170, and 357 mg/kg). All the groups orally administered 3,4-DCA or corn oil, once a day for 4 weeks. The testicle tissue was homogenized in a 0.1 mol/L potassium phosphate buffer (0.1 mol/L, pH 7.2). The crude homogenate was centrifuged at 6000 rpm for 5 min at 4 degrees C. The supernatant obtained was used as an enzyme extract for determination of the enzyme activities.

Results: Compared with the control, the activities of ALP, ACP, and SDH were increased significantly at a lower level of 3,4-DCA, and decreased at a higher level of 3, 4-DCA, whreas the activities of LDH, LDH-X, and G6PDH were inhibited significantly with the increased 3,4-DCA concentration. Organ coefficient "organ weight/total body weight x 100" of testis, liver, and spleen increased significantly with the increased 3,4-DCA concentration. These results suggest that 3,4-DCA toxicity to the male reproductive system was associated with the activities of testicular enzymes which are the sensitive biochemical endpoints reflecting 3,4-DCA toxicity to the male reproductive system.

Conclusion: 3,4-DCA has toxicity to the reproductive system in male rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds / toxicity*
  • Animals
  • Biomarkers / analysis
  • Body Weight / drug effects
  • Male
  • Organ Size / drug effects
  • Rats
  • Rats, Wistar
  • Testis / drug effects*
  • Testis / enzymology
  • Toxicity Tests

Substances

  • Aniline Compounds
  • Biomarkers
  • 3,4-dichloroaniline