Treatment of injured neurons with bone marrow stem cells cotransfected by hTERT and Ad-BDNF in vitro

J Mol Neurosci. 2009 Jul;38(3):265-72. doi: 10.1007/s12031-009-9208-5. Epub 2009 May 21.

Abstract

The purpose of this paper is to study the anti-injury effect of bone marrow stromal cells (BMSCs) transfected by human telomerase reverse transcriptase (hTERT) and adenovirous brain-derived neurotrophic factor (Ad-BDNF) in injured neurons. Rat injured neurons were induced in vitro and then cocultured with rBMSCs transfected by hTERT and Ad-BDNF. The anti-injury effect of rBMSCs transfected by hTERT and Ad-BDNF was detected by Western blot for neural apoptosis gene MAP2 and NF-kappaB and lactic dehydrogenase released by injured neurons. Our data demonstrated that graft rBMSCs transfected by hTERT and Ad-BDNF could increase the expression of BDNF in injured neurons and alleviate apoptosis of the injured neurons. According to these data, BDNF-BMSCs demonstrate a tendency to be protection against neuronal death caused by apoptosis which would last a long time after neural injury. The data also show the potential of BDNF-BMSCs as a promising therapeutic strategy that warrants further investigation in patients with traumatic brain injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Adenoviridae / metabolism
  • Animals
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / physiology*
  • Bone Marrow Transplantation*
  • Brain-Derived Neurotrophic Factor / genetics
  • Brain-Derived Neurotrophic Factor / metabolism*
  • Cell Shape
  • Cells, Cultured
  • Coculture Techniques
  • Humans
  • Nerve Regeneration / physiology
  • Neurons* / pathology
  • Neurons* / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Stem Cells / cytology
  • Stem Cells / physiology*
  • Telomerase / genetics
  • Telomerase / metabolism*

Substances

  • Brain-Derived Neurotrophic Factor
  • TERT protein, human
  • Telomerase