DIXDC1 co-localizes and interacts with gamma-tubulin in HEK293 cells

Cell Biol Int. 2009 Jun;33(6):697-701. doi: 10.1016/j.cellbi.2009.04.001. Epub 2009 Apr 16.

Abstract

DIXDC1 is a Dishevelled-Axin (DIX) domain-containing protein involved in neural development and Wnt signaling pathway. Besides the DIX domain, DIXDC1 also contains a coiled-coil domain (MTH domain), which is a common feature of centrosomal proteins. We have demonstrated that exogenously expressed GFP-tag fused DIXDC1 co-localize with gamma-tubulin both at interphase and mitotic phase in HEK293 cells. By immunostaining with anti-DIXDC1 and anti-gamma-tubulin antibody, endogenous DIXDC1 was also co-localized with gamma-tubulin at the centrosomes in HEK293 cells. We confirmed this interaction of DIXDC1 with gamma-tubulin by co-immunoprecipitation. The findings suggest that DIXDC1 might play an important role in chromosome segregation and cell cycle regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Centrosome / metabolism
  • Chromosome Segregation
  • Humans
  • Immunoprecipitation
  • Interphase
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Microfilament Proteins / metabolism*
  • Mitosis
  • Signal Transduction
  • Tubulin / metabolism*

Substances

  • DIXDC1 protein, human
  • Intracellular Signaling Peptides and Proteins
  • Microfilament Proteins
  • Tubulin

Associated data

  • RefSeq/NM_001037954