The PcG protein hPc2 interacts with the N-terminus of histone demethylase JARID1B and acts as a transcriptional co-repressor

BMB Rep. 2009 Mar 31;42(3):154-9. doi: 10.5483/bmbrep.2009.42.3.154.

Abstract

JARID1B (jumonji AT rich interactive domain 1B) is a large nuclear protein that is highly expressed in breast cancers and is proposed to function as a repressor of gene expression. In this paper, a phage display screen using the N-terminus of JARID1B as bait identified one of the JARID1B interacting proteins, namely PcG protein (Polycomb group) hPc2. We demonstrated that the C-terminal region, including the COOH box, was required for the interaction with the N-terminus of JARID1B. In a reporter assay system, co-expression of JARID1B with hPc2 significantly enhanced the transcriptional repression. These results support a role for hPc2 acting as a transcriptional co-repressor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Jumonji Domain-Containing Histone Demethylases
  • Ligases
  • Nuclear Proteins / chemistry*
  • Nuclear Proteins / metabolism*
  • Oxidoreductases, N-Demethylating / chemistry*
  • Oxidoreductases, N-Demethylating / metabolism*
  • Peptide Library
  • Polycomb-Group Proteins
  • Protein Binding
  • Repressor Proteins / chemistry
  • Repressor Proteins / metabolism*
  • Transcription, Genetic*
  • Ubiquitin-Protein Ligases

Substances

  • DNA-Binding Proteins
  • Nuclear Proteins
  • Peptide Library
  • Polycomb-Group Proteins
  • Repressor Proteins
  • Jumonji Domain-Containing Histone Demethylases
  • KDM5B protein, human
  • Oxidoreductases, N-Demethylating
  • Ubiquitin-Protein Ligases
  • Ligases
  • CBX4 protein, human