Determination of the 5' exon structure of the human cardiac alpha-myosin heavy chain gene

Biochem Biophys Res Commun. 1991 Sep 30;179(3):1255-8. doi: 10.1016/0006-291x(91)91707-j.

Abstract

We have deduced the exon structure of the 5' untranslated region of the human cardiac alpha-myosin heavy chain gene by cloning a cDNA for this region using the polymerase chain reaction. Comparison of the cDNA and genomic DNA sequences demonstrates that the 5' non-coding region of the alpha-myosin heavy chain gene is interrupted by two introns of 645 and 337 nucleotides. Secondly we have identified the transcriptional start-site by primer extension, corroborating the previous putative assignment for the alpha-myosin heavy chain promoter based on comparisons between the rat and human genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • Exons*
  • Genes
  • Heart / physiology*
  • Humans
  • Molecular Sequence Data
  • Myosins / genetics*
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Rats
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • Oligodeoxyribonucleotides
  • DNA
  • Myosins

Associated data

  • GENBANK/M74785
  • GENBANK/M74786
  • GENBANK/M74787
  • GENBANK/M74788
  • GENBANK/M74790
  • GENBANK/M81108
  • GENBANK/M81109
  • GENBANK/S57525
  • GENBANK/S57535
  • GENBANK/X56181