Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase

Rakesh N Veedu; Birte Vester; Jesper Wengel

doi:10.1039/b819946a

Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase

Org Biomol Chem. 2009 Apr 7;7(7):1404-9. doi: 10.1039/b819946a. Epub 2009 Feb 24.

Authors

Rakesh N Veedu¹, Birte Vester, Jesper Wengel

Affiliation

¹ Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, Odense M, Denmark.

PMID: 19300826
DOI: 10.1039/b819946a

Abstract

Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Catalysis
DNA / chemistry*
DNA / metabolism*
DNA-Directed DNA Polymerase / metabolism*
Molecular Sequence Data
Oligonucleotides / chemistry*
Oligonucleotides / metabolism*

Substances

Oligonucleotides
locked nucleic acid
DNA
DNA-Directed DNA Polymerase