Identification and characterization of a novel cytoskeleton-associated pp60src substrate

Mol Cell Biol. 1991 Oct;11(10):5113-24. doi: 10.1128/mcb.11.10.5113-5124.1991.

Abstract

Transformation of cells by the src oncogene results in elevated tyrosine phosphorylation of two related proteins, p80 and p85 (p80/85). Immunostaining with specific monoclonal antibodies revealed a striking change of subcellular localization of p80/85 in src-transformed cells. p80/85 colocalizes with F-actin in peripheral extensions of normal cells and rosettes (podosomes) of src-transformed cells. Sequence analysis of cDNA clones encoding p80/85 revealed an amino-terminal domain composed of six copies of a direct tandem repeat, each repeat containing 37 amino acids, a carboxyl-terminal SH3 domain, and an interdomain region composed of a highly charged acidic region and a region rich in proline, serine, and threonine. The multidomain structure of p80/85 and its colocalization with F-actin in normal and src-transformed cells suggest that these proteins may associate with components of the cytoskeleton and contribute to organization of cell structure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Avian Proteins*
  • Base Sequence
  • Blotting, Northern
  • Blotting, Western
  • Cell Transformation, Viral / genetics
  • Cell Transformation, Viral / physiology*
  • Cells, Cultured
  • Chick Embryo
  • Cloning, Molecular
  • Cytoskeletal Proteins / chemistry*
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / metabolism
  • Cytoskeleton / metabolism*
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Oncogene Protein pp60(v-src) / metabolism*
  • Phosphorylation
  • Protein Conformation
  • Repetitive Sequences, Nucleic Acid / genetics
  • Serine / metabolism
  • Threonine / metabolism
  • Tyrosine / metabolism

Substances

  • Avian Proteins
  • Cytoskeletal Proteins
  • p80 protein, chicken
  • cytoskeletal p85 protein, chicken
  • Threonine
  • Tyrosine
  • Serine
  • Oncogene Protein pp60(v-src)

Associated data

  • GENBANK/M64827
  • GENBANK/M73024
  • GENBANK/M73025
  • GENBANK/M73026
  • GENBANK/M73027
  • GENBANK/M73705
  • GENBANK/S43616
  • GENBANK/S43620
  • GENBANK/S57521
  • GENBANK/X58858