Abstract
Two inhibitors of Taq DNA polymerase were isolated from the marine red alga Symphyocladia latiuscula. The inhibitors were purified by methanol extraction, molecular fractionation below 3000 MW and reverse-phase HPLC. The purified compound SL-1 containing three bromines was identified as 2,3,6-tribromo-4,5-dihydroxybenzyl alcohol (C7H5Br3O3: MW374) by NMR and MS analyses. The purified compound SL-2 was identified as 2,3, 6-tribromo-4,5-dihydroxybenzyl methyl ether(C8H7Br3O3: MW388). In a 25-microl reaction mixture containing 1.5 units of Taq DNA polymerase, the enzyme was completely inhibited by 0.5 microg SL-1 or 5 microg SL-2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Chromatography, High Pressure Liquid
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Electrophoresis, Agar Gel
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / isolation & purification*
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Enzyme Inhibitors / pharmacology*
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Ethers
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Hydrocarbons, Brominated / chemistry
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Hydrocarbons, Brominated / isolation & purification*
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Hydrocarbons, Brominated / pharmacology*
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Methanol / chemistry
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Molecular Weight
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Polymerase Chain Reaction
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Rhodophyta / chemistry*
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Spectrum Analysis
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Taq Polymerase / antagonists & inhibitors*
Substances
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2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether
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Enzyme Inhibitors
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Ethers
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Hydrocarbons, Brominated
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Taq Polymerase
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Methanol