The aim of this study was to examine the relationships between group cohesion and the perceived culture and desired culture of work teams. Two separate studies were carried out with a time interval of one year. The first study had a sample of 50 work teams belonging to different organizations and the second study had a sample of 75 work teams. We used Lindell's index of agreement to estimate the culture and cohesion variables. Multiple and hierarchical regression analysis in both studies confirmed our initial hypothesis, showing that perceived and desired culture were positively related to group cohesion, and that culture gap had a negative association with cohesion. To a lesser extent, control variables such as job tenure and gender had a positive relationship with cohesion. The implications of these results are discussed along with the importance of considering the group level as analysis.