Selective recognition of unnatural imidazopyridopyrimidine:naphthyridine base pairs consisting of four hydrogen bonds by the Klenow fragment

Noriaki Minakawa; Shintaro Ogata; Mayumi Takahashi; Akira Matsuda

doi:10.1021/ja807391g

Selective recognition of unnatural imidazopyridopyrimidine:naphthyridine base pairs consisting of four hydrogen bonds by the Klenow fragment

J Am Chem Soc. 2009 Feb 11;131(5):1644-5. doi: 10.1021/ja807391g.

Authors

Noriaki Minakawa¹, Shintaro Ogata, Mayumi Takahashi, Akira Matsuda

Affiliation

¹ Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

PMID: 19146369
DOI: 10.1021/ja807391g

Abstract

In this work, we investigated how thermally stable ImO(N):NaN(O) and ImN(O):NaO(N) pairs are recognized by the Klenow fragment (KF). As a result, these complementary base pairs, especially the ImN(O):NaO(N) pair, were recognized selectively due to the four hydrogen bonds between the nucleobases and the shape complementarity of the Im:Na pair similar to the purine:pyrimidine base pair.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pairing
DNA Polymerase I / chemistry*
Hydrogen Bonding
Imidazoles / chemistry
Kinetics
Naphthyridines / chemistry*
Nucleotides / chemistry
Pyrimidines / chemistry*

Substances

Imidazoles
Naphthyridines
Nucleotides
Pyrimidines
DNA Polymerase I