Contribution of vraSR and graSR point mutations to vancomycin resistance in vancomycin-intermediate Staphylococcus aureus

Longzhu Cui; Hui-min Neoh; Mitsutaka Shoji; Keiichi Hiramatsu

doi:10.1128/AAC.01173-08

Contribution of vraSR and graSR point mutations to vancomycin resistance in vancomycin-intermediate Staphylococcus aureus

Antimicrob Agents Chemother. 2009 Mar;53(3):1231-4. doi: 10.1128/AAC.01173-08. Epub 2009 Jan 5.

Authors

Longzhu Cui¹, Hui-min Neoh, Mitsutaka Shoji, Keiichi Hiramatsu

Affiliation

¹ Department of Bacteriology, Faculty of Medicine, Juntendo University, Tokyo, Japan. longzhu@juntendo.ac.jp

Abstract

We describe here the genetic analysis of a vancomycin-susceptible Staphylococcus aureus (VSSA) strain, Mu50Omega, a strain related to vancomycin-intermediate S. aureus (VISA) strain Mu50. Using a combination of Mu50Omega whole-genome sequencing and genome engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to VISA after the mutated vraS and graR genes of Mu50 were engineered into Mu50Omega.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / chemistry
Bacterial Proteins / genetics*
Base Sequence
Chromosome Walking
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics*
Genetic Engineering
Genome, Bacterial
Humans
Microbial Sensitivity Tests
Molecular Sequence Data
Point Mutation*
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Staphylococcus aureus / drug effects
Staphylococcus aureus / genetics*
Staphylococcus aureus / growth & development
Staphylococcus aureus / metabolism
Vancomycin / pharmacology
Vancomycin Resistance / genetics*

Substances

Anti-Bacterial Agents
Bacterial Proteins
DNA-Binding Proteins
VraS protein, Staphylococcus aureus
Vancomycin