Abstract
Induced pluripotent stem (iPS) cells can be generated from somatic cells by transduction with several transcription factors in mouse and human. However, direct reprogramming in other species has not been reported. Here, we generated monkey iPS cells by retrovirus-mediated introduction of monkey transcription factors OCT4, SOX2, KLF4, and c-MYC.
Publication types
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Letter
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biomarkers / analysis
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Biomarkers / metabolism
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Cell Culture Techniques / methods
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Cell Differentiation / drug effects
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Cell Differentiation / genetics*
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Cell Proliferation / drug effects
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Cell Shape / drug effects
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Cell Shape / genetics
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Cells, Cultured
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Culture Media / chemistry
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Culture Media / pharmacology
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Embryonic Development / genetics
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / metabolism*
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Gene Expression Regulation / genetics
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Genetic Vectors / genetics
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Germ Layers / cytology
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Germ Layers / metabolism
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Kruppel-Like Factor 4
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Kruppel-Like Transcription Factors / genetics
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Macaca mulatta / anatomy & histology
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Macaca mulatta / physiology*
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Male
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Octamer Transcription Factor-3 / genetics
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Pluripotent Stem Cells / cytology
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Pluripotent Stem Cells / metabolism*
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Proto-Oncogene Proteins c-myc / genetics
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Retroviridae / genetics
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SOXB1 Transcription Factors / genetics
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Transcription Factors / genetics*
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Transduction, Genetic / methods*
Substances
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Biomarkers
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Culture Media
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KLF4 protein, human
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Klf4 protein, mouse
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Kruppel-Like Factor 4
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Kruppel-Like Transcription Factors
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Octamer Transcription Factor-3
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POU5F1 protein, human
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Proto-Oncogene Proteins c-myc
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SOX2 protein, human
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SOXB1 Transcription Factors
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Transcription Factors