Protein kinase Cdelta expression in breast cancer as measured by real-time PCR, western blotting and ELISA

Br J Cancer. 2008 Nov 18;99(10):1644-50. doi: 10.1038/sj.bjc.6604728. Epub 2008 Oct 28.

Abstract

The protein kinase C (PKC) family of genes encode serine/threonine kinases that regulate proliferation, apoptosis, cell survival and migration. Multiple isoforms of PKC have been described, one of which is PKCdelta. Currently, it is unclear whether PKCdelta is involved in promoting or inhibiting cancer formation/progression. The aim of this study was therefore to investigate the expression of PKCdelta in human breast cancer and relate its levels to multiple parameters of tumour progression. Protein kinase Cdelta expression at the mRNA level was measured using real-time PCR (n=208) and at protein level by both immunoblotting (n=94) and ELISA (n=98). Following immunoblotting, two proteins were identified, migrating with molecular masses of 78 and 160 kDa. The 78 kDa protein is likely to be the mature form of PKCdelta but the identity of the 160 kDa form is unknown. Levels of both these proteins correlated weakly but significantly with PKCdelta concentrations determined by ELISA (for the 78 kDa form, r=0.444, P<0.005, n=91 and for the 160 kDa form, r=0.237, P=0.023, n=91) and with PKCdelta mRNA levels (for the 78 kDa form, r=0.351, P=0.001, n=94 and for the 160 kDa form, r=0.216, P=0.037, n=94). Protein kinase Cdelta mRNA expression was significantly higher in oestrogen receptor (ER)-positive compared with ER-negative tumours (P=0.007, Mann-Whitney U-test). Increasing concentrations of PKCdelta mRNA were associated with reduced overall patient survival (P=0.004). Our results are consistent with a role for PKCdelta in breast cancer progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western*
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism*
  • Disease Progression
  • Enzyme-Linked Immunosorbent Assay*
  • Female
  • Humans
  • Middle Aged
  • Polymerase Chain Reaction
  • Protein Kinase C-delta / biosynthesis*
  • Protein Kinase C-delta / genetics
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction*
  • Survival Analysis

Substances

  • RNA, Messenger
  • Protein Kinase C-delta