Sequencing and bacterial expression of a novel murine alpha interferon gene

Y Wang; Z Wang; M Zhou; J Cai; L Sun; X Liu; B L Daugherty; S Pestka

doi:10.1007/BF02879088

Sequencing and bacterial expression of a novel murine alpha interferon gene

Sci China C Life Sci. 1997 Jun;40(3):277-83. doi: 10.1007/BF02879088.

Authors

Y Wang¹, Z Wang, M Zhou, J Cai, L Sun, X Liu, B L Daugherty, S Pestka

Affiliation

¹ Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031, Shanghai, China.

PMID: 18726328
DOI: 10.1007/BF02879088

Abstract

A murine new alpha interferon gene (mIFN-alphaB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the P(L) promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-alphaB has been accepted by GENEBANK.