Abstract
We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading frame in the Epstein-Barr virus, BCRFI. hIL-10 and the BCRFI product inhibit cytokine synthesis by activated human peripheral blood mononuclear cells and by a mouse Th1 clone. Both hIL-10 and mouse IL-10 sustain the viability of a mouse mast cell line in culture, but BCRFI lacks comparable activity in this assay, suggesting that BCRFI may have conserved only a subset of hIL-10 activities.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Line
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Cloning, Molecular
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DNA / genetics
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DNA / isolation & purification
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Granulocyte-Macrophage Colony-Stimulating Factor / antagonists & inhibitors
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Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
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Herpesvirus 4, Human / genetics*
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Humans
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Interferon-gamma / antagonists & inhibitors
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Interferon-gamma / biosynthesis
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Interleukin-10
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Interleukins / genetics*
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Interleukins / pharmacology
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Leukocytes, Mononuclear / immunology
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Mice
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Molecular Sequence Data
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Open Reading Frames*
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Polymerase Chain Reaction
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RNA, Messenger / genetics
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Recombinant Proteins / pharmacology
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Sequence Homology, Nucleic Acid
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Transfection
Substances
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Interleukins
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RNA, Messenger
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Recombinant Proteins
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Interleukin-10
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Interferon-gamma
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Granulocyte-Macrophage Colony-Stimulating Factor
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DNA
Associated data
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GENBANK/M57627
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GENBANK/S74646
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GENBANK/S74648
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GENBANK/S74650
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GENBANK/S74653
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GENBANK/S74655
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GENBANK/S74658
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GENBANK/S74661
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GENBANK/S74663
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GENBANK/X12824