Structure and functional expression of a human interleukin-8 receptor

Science. 1991 Sep 13;253(5025):1278-80. doi: 10.1126/science.1840701.

Abstract

Interleukin-8 (IL-8) is a member of a family of pro-inflammatory cytokines. Although the best characterized activities of IL-8 include the chemoattraction and activation of neutrophils, other members of this family have a wide range of specific actions including the chemotaxis and activation of monocytes, the selective chemotaxis of memory T cells, the inhibition of hematopoietic stem cell proliferation, and the induction of neutrophil infiltration in vivo. A complementary DNA encoding the IL-8 receptor from human neutrophils has now been isolated. The amino acid sequence shows that the receptor is a member of the superfamily of receptors that couple to guanine nucleotide binding proteins (G proteins). The sequence is 29% identical to that of receptors for the other neutrophil chemoattractants, fMet-Leu-Phe and C5a. Mammalian cells transfected with the IL-8 receptor cDNA clone bind IL-8 with high affinity and respond specifically to IL-8 by transiently mobilizing calcium. The IL-8 receptor may be part of a subfamily of related G protein-coupled receptors that transduce signals for the IL-8 family of pro-inflammatory cytokines.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cloning, Molecular
  • DNA Probes
  • Humans
  • Interleukin-8 / metabolism*
  • Kinetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Plasmids
  • RNA, Messenger / genetics
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Receptors, Interleukin-8A
  • Recombinant Proteins / metabolism
  • Sequence Homology, Nucleic Acid
  • Transfection

Substances

  • DNA Probes
  • Interleukin-8
  • RNA, Messenger
  • Receptors, Immunologic
  • Receptors, Interleukin-8A
  • Recombinant Proteins

Associated data

  • GENBANK/M61418
  • GENBANK/M61419
  • GENBANK/M61420
  • GENBANK/M61421
  • GENBANK/M61422
  • GENBANK/M61423
  • GENBANK/M61424
  • GENBANK/M61425
  • GENBANK/M61426
  • GENBANK/M68932