siRNA-mediated inhibition of phosphatidylinositol glycan Class B (PIGB) confers resistance to methylmercury in HEK293 cells

Gi-Wook Hwang; Tatsuya Hayashi; Kayoko Kita; Tsutomu Takahashi; Shusuke Kuge; Akira Naganuma

doi:10.2131/jts.32.581

siRNA-mediated inhibition of phosphatidylinositol glycan Class B (PIGB) confers resistance to methylmercury in HEK293 cells

J Toxicol Sci. 2007 Dec;32(5):581-3. doi: 10.2131/jts.32.581.

Authors

Gi-Wook Hwang¹, Tatsuya Hayashi, Kayoko Kita, Tsutomu Takahashi, Shusuke Kuge, Akira Naganuma

Affiliation

¹ Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.

PMID: 18198489
DOI: 10.2131/jts.32.581

Abstract

We performed functional gene screening, using a siRNA library targeting 8,500 human genes, to identify proteins that are involved in the susceptibility of cells to methylmercury. Screening revealed that downregulation of the gene for phosphatidylinositol glycan class B (PIGB) by siRNA confers resistance to methylmercury in HEK293 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drug Resistance / genetics*
Humans
Mannosyltransferases / genetics*
Mannosyltransferases / metabolism
Methylmercury Compounds / toxicity*
RNA Interference*
RNA, Small Interfering / metabolism*
Transfection

Substances

Methylmercury Compounds
RNA, Small Interfering
Mannosyltransferases
PIGB protein, human
methylmercuric chloride