Abstract
Bioprospecting for novel antimicrobials increasingly relies on extremely small samples unsuitable for conventional bulk extraction and assay. We developed a microtitre plate assay for minimal amounts of test materials which is rapid, extremely sensitive, allows time-course analysis and reduces false negatives. Developed for the analyses of antimicrobial sensitivity and resistance, the technique is appropriate for assays where source materials are scarce.
MeSH terms
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Ampicillin / pharmacology
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Animals
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Anti-Bacterial Agents / analysis*
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Anti-Bacterial Agents / pharmacology*
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Drug Resistance, Bacterial / genetics
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Escherichia coli / drug effects*
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Escherichia coli / genetics
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Escherichia coli / growth & development
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Inhibitory Concentration 50
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Insecta / chemistry
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Microbial Sensitivity Tests / methods*
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Plasmids
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Reproducibility of Results
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Sensitivity and Specificity
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Streptomycin / pharmacology
Substances
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Anti-Bacterial Agents
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Escherichia coli Proteins
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Ampicillin
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Streptomycin