DNA polymerase I is not required for replication of linear chromosomes in streptomyces

Tzu-Wen Huang; Carton W Chen

doi:10.1128/JB.01335-07

DNA polymerase I is not required for replication of linear chromosomes in streptomyces

J Bacteriol. 2008 Jan;190(2):755-8. doi: 10.1128/JB.01335-07. Epub 2007 Nov 9.

Authors

Tzu-Wen Huang¹, Carton W Chen

Affiliation

¹ Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan.

Abstract

Both polA (encoding DNA polymerase I; Pol I) and a paralog were deleted from Streptomyces strains. Despite the UV sensitivity and slow growth caused by the DeltapolA mutation, the double mutant was viable. Thus, in contrast to a previous postulate, Pol I and its paralog are not essential for replication of Streptomyces chromosomes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Bacterial / metabolism*
DNA Polymerase I / genetics
DNA Polymerase I / metabolism*
DNA, Bacterial / biosynthesis*
Gene Deletion
Microbial Viability
Mutagenesis, Insertional
Streptomyces / genetics
Streptomyces / growth & development
Streptomyces / metabolism*
Streptomyces / radiation effects
Ultraviolet Rays

Substances

DNA, Bacterial
DNA Polymerase I