Abstract
The partial pol gene was analyzed to determine circulating HIV-1 subtypes and the prevalence of drug resistance among recently infected injecting drug users in the west of China. Phylogenetic and bootscanning analyses showed that all 25 sequences belonged to CRF07_BC. No major drug resistance mutations were found, while the CRF07_BC sequences harbored a few highly prevalent polymorphic positions in both the protease and reverse transcriptase regions. It is notable that D60E, L63P and I93L substitutions, which were more common in HIV-1 isolates from PI-treated than untreated patients, were present in most of the isolates. Compared with subtype B, the CRF07_BC strains had a decreased genetic barrier for the V106M mutation, which is selected by efavirenz and leads to high-level resistance to all present nonnucleoside RT inhibitors. Further studies are needed to determine the impact of genetic features of CRF07-BC on antitretroviral susceptibility and evolution of drug resistance mutations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-HIV Agents / pharmacology
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China
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Drug Resistance, Viral / genetics*
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HIV Infections / complications
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HIV Infections / virology
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HIV Protease / genetics*
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HIV Reverse Transcriptase / genetics*
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HIV-1 / drug effects
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HIV-1 / enzymology
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HIV-1 / genetics*
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Humans
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Molecular Sequence Data
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Polymorphism, Genetic
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Prevalence
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Substance Abuse, Intravenous / complications*
Substances
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Anti-HIV Agents
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HIV Reverse Transcriptase
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HIV Protease
Associated data
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GENBANK/EF432322
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GENBANK/EF432323
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GENBANK/EF432324
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GENBANK/EF432325
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