Abstract
The expression of platelet-derived growth factor (PDGF), transforming growth factor (TGF)-beta, and connective tissue growth factor (CTGF) and the effect of imatinib, an agent inhibiting PDGF receptors, were assessed in a porcine bronchial transplantation model of obliterative bronchiolitis (OB). Up-regulation of PDGF-A, PDGF receptors alpha and beta, and TGF-beta expression occurred in allografts, whereas PDGF-B and CTGF expression was similar in allo- and autografts. Imatinib modified the inflammatory responses and expression patterns of PDGF-A and PDGF receptors. This study further confirms PDGF and TGF-beta as mediators of OB and supports the concept of the importance of the pathways signaled through PDGF receptors in post-transplant OB.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Benzamides
-
Bronchi / drug effects
-
Bronchi / metabolism*
-
Bronchi / pathology
-
Bronchi / transplantation
-
Bronchiolitis Obliterans / metabolism*
-
Bronchiolitis Obliterans / pathology
-
CD4-Positive T-Lymphocytes / pathology
-
CD8-Positive T-Lymphocytes / pathology
-
Connective Tissue Growth Factor
-
Disease Models, Animal
-
Graft Rejection / metabolism*
-
Graft Rejection / pathology
-
Imatinib Mesylate
-
Immediate-Early Proteins / metabolism*
-
Immunohistochemistry
-
Intercellular Signaling Peptides and Proteins / metabolism*
-
Macrophages / pathology
-
Piperazines / pharmacology
-
Platelet-Derived Growth Factor / metabolism*
-
Protein Kinase Inhibitors / pharmacology
-
Proto-Oncogene Proteins c-sis / metabolism*
-
Pyrimidines / pharmacology
-
Receptor, Platelet-Derived Growth Factor alpha / antagonists & inhibitors
-
Receptor, Platelet-Derived Growth Factor alpha / metabolism
-
Receptor, Platelet-Derived Growth Factor beta / antagonists & inhibitors
-
Receptor, Platelet-Derived Growth Factor beta / metabolism
-
Signal Transduction
-
Sus scrofa
-
Time Factors
-
Transforming Growth Factor beta / metabolism*
-
Transplantation, Autologous
-
Transplantation, Homologous
Substances
-
Benzamides
-
Immediate-Early Proteins
-
Intercellular Signaling Peptides and Proteins
-
Piperazines
-
Platelet-Derived Growth Factor
-
Protein Kinase Inhibitors
-
Proto-Oncogene Proteins c-sis
-
Pyrimidines
-
Transforming Growth Factor beta
-
platelet-derived growth factor A
-
Connective Tissue Growth Factor
-
Imatinib Mesylate
-
Receptor, Platelet-Derived Growth Factor alpha
-
Receptor, Platelet-Derived Growth Factor beta