Abstract
We isolated the glycolipids fraction from spinach (Spinacia oleracea L.) and found that the fraction inhibited the activities of prokaryotic DNA polymerase I from Escherichia coli (E. coli) and cell growth of E. coli. The fraction contained mainly three glycolipids, monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG) and sulfoquinovosyl diacylglycerol (SQDG), and purified SQDG inhibited these activities, however, purified MGDG and DGDG had no influence. In the tested strains of E. coli, SQDG inhibited the cell proliferation of the JM109 strain. It could be considered that a SQDG-containing thylakoid membrane in plant chloroplasts might have anti-bacterial activity.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Proliferation / drug effects*
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DNA Polymerase I / antagonists & inhibitors*
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DNA Polymerase I / metabolism
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / isolation & purification
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Enzyme Inhibitors / pharmacology
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Escherichia coli / cytology*
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Escherichia coli / drug effects
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Escherichia coli / enzymology*
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Glycolipids / chemistry
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Glycolipids / isolation & purification
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Glycolipids / pharmacology*
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Growth Inhibitors / chemistry
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Growth Inhibitors / isolation & purification
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Growth Inhibitors / pharmacology*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Prokaryotic Cells / drug effects
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Prokaryotic Cells / enzymology
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Spinacia oleracea
Substances
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Enzyme Inhibitors
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Glycolipids
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Growth Inhibitors
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Plant Extracts
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sulfoquinovosyl diglyceride
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DNA Polymerase I