Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes

Biochem Biophys Res Commun. 2007 Aug 3;359(3):729-34. doi: 10.1016/j.bbrc.2007.05.173. Epub 2007 Jun 4.

Abstract

We performed the screening to find the novel host factors affecting human immunodeficiency virus type-1 (HIV-1) replication using the siRNA mini-library consisted with 257 siRNAs directed against cellular genes. J111 cells, a human acute monocytic leukemia cell line, were transfected with individual siRNA, followed by either infected or transfected with the HIV-1 molecular clone with luciferase reporter gene in 96-well plate format. The results showed that six siRNAs significantly enhanced the HIV-1 replication in J111 cells, indicating that the target cellular genes of those siRNAs may negatively regulate HIV-1 replication in normal cell culture condition. We also discuss the possible mechanisms by which those cellular proteins regulate viral replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Gene Expression Regulation
  • Gene Library*
  • HIV-1 / physiology*
  • Humans
  • RNA, Small Interfering / genetics*
  • Suppressor Factors, Immunologic / genetics*
  • Suppressor Factors, Immunologic / metabolism*
  • Virus Replication / physiology*

Substances

  • RNA, Small Interfering
  • Suppressor Factors, Immunologic