Abstract
In contrast to solid tumors, leukemic blasts frequently present both Hsp70 and HLA-E on their cell surface and thereby present activating and inhibitory signals to CD94(+) NK cells. In the first 12 months after stem cell transplantation (SCT) CD94(+) NK cells clearly dominate over CD3(+)/CD16(-)/56(-) T and CD3(+)/CD16(+)/56(+) NK-like T cells. An incubation of post-SCT-derived peripheral blood lymphocytes with the Hsp70 peptide TKD and IL-15 enhances the cell surface density of CD56/CD94 and initiates the cytolytic activity of NK cells against Hsp70/HLA-E double-positive autologous and allogeneic leukemic blasts. Hsp70 was identified as the target structure for TKD-activated NK cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / metabolism
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Cytotoxicity, Immunologic
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Enzyme-Linked Immunosorbent Assay
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Flow Cytometry
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HLA Antigens / physiology
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HLA-E Antigens
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HSP70 Heat-Shock Proteins / physiology*
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Histocompatibility Antigens Class I / physiology
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Humans
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Interleukin-15 / metabolism
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Killer Cells, Natural / immunology*
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Leukemia / immunology
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Leukemia / pathology*
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Leukemia, Lymphocytic, Chronic, B-Cell / immunology
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Leukemia, Lymphocytic, Chronic, B-Cell / pathology
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / immunology
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology
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Leukemia, Myeloid, Acute / immunology
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Leukemia, Myeloid, Acute / pathology
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Leukocytes, Mononuclear / drug effects
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / metabolism
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Middle Aged
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Peptide Fragments / physiology*
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / immunology
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
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Stem Cell Transplantation*
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Tumor Cells, Cultured
Substances
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Antigens, CD
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HLA Antigens
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HSP70 Heat-Shock Proteins
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Histocompatibility Antigens Class I
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Interleukin-15
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Peptide Fragments