Increased single nucleotide discrimination in arrayed primer elongation by 4'C-modified primer probes

Jens Gaster; Gopinath Rangam; Andreas Marx

doi:10.1039/b616129d

Increased single nucleotide discrimination in arrayed primer elongation by 4'C-modified primer probes

Chem Commun (Camb). 2007 May 7:(17):1692-4. doi: 10.1039/b616129d. Epub 2007 Jan 2.

Authors

Jens Gaster¹, Gopinath Rangam, Andreas Marx

Affiliation

¹ Universität Konstanz, Fachbereich Chemie, Universitätsstr. 10, 78457 Konstanz, Germany.

PMID: 17457410
DOI: 10.1039/b616129d

Abstract

Herein we describe the beneficial impact of immobilized 4'C-modified primer probes on detecting single nucleotide variations in arrayed primer extension by a DNA polymerase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Carbon / chemistry*
DNA Primers / chemistry*
DNA Primers / genetics
DNA Primers / metabolism*
Molecular Structure
Nucleotides / metabolism*
Oligonucleotide Array Sequence Analysis

Substances

DNA Primers
Nucleotides
Carbon