Mobilization of pro-inflammatory lipids in obese Plscr3-deficient mice

Genome Biol. 2007;8(3):R38. doi: 10.1186/gb-2007-8-3-r38.

Abstract

Background: The obesity epidemic has prompted the search for candidate genes capable of influencing adipose function. One such candidate, that encoding phospholipid scramblase 3 (PLSCR3), was recently identified, as genetic deletion of it led to lipid accumulation in abdominal fat pads and changes characteristic of metabolic syndrome. Because adipose tissue is increasingly recognized as an endocrine organ, capable of releasing small molecules that modulate disparate physiological processes, we examined the plasma from wild-type, Plscr1-/-, Plscr3-/- and Plscr1&3-/- mice. Using an untargeted comprehensive metabolite profiling approach coupled with targeted gene expression analyses, the perturbed biochemistry and functional redundancy of PLSCR proteins was assessed.

Results: Nineteen metabolites were differentially and similarly regulated in both Plscr3-/- and Plscr1&3-/- animals, of which five were characterized from accurate mass, tandem mass spectrometry data and their correlation to the Metlin database as lysophosphatidylcholine (LPC) species enriched with C16:1, C18:1, C20:3, C20:5 and C22:5 fatty acids. No significant changes in the plasma metabolome were detected upon elimination of PLSCR1, indicating that increases in pro-inflammatory lipids are specifically associated with the obese state of Plscr3-deficient animals. Correspondingly, increases in white adipose lipogenic gene expression confirm a role for PLSCR3 in adipose lipid metabolism.

Conclusion: The untargeted profiling of circulating metabolites suggests no detectable functional redundancies between PLSCR proteins; however, this approach simultaneously identified previously unrecognized lipid metabolites that suggest a novel molecular link between obesity, inflammation and the downstream consequences associated with PLSCR3-deficiency.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adipose Tissue, White / metabolism
  • Animals
  • Fatty Acids / analysis
  • Gene Expression Profiling
  • Inflammation / etiology*
  • Lipid Metabolism*
  • Lysophosphatidylcholines / chemistry
  • Mice
  • Mice, Knockout
  • Obesity / etiology*
  • Phospholipid Transfer Proteins / deficiency*
  • Phospholipid Transfer Proteins / physiology
  • Tandem Mass Spectrometry

Substances

  • Fatty Acids
  • Lysophosphatidylcholines
  • Phospholipid Transfer Proteins
  • Plscr3 protein, mouse