Regional localization of the human G protein alpha i2 (GNAI2) gene: assignment to 3p21 and a related sequence (GNAI2L) to 12p12-p13

Genomics. 1992 Jan;12(1):125-9. doi: 10.1016/0888-7543(92)90414-n.

Abstract

Gi alpha proteins, members of the G protein signal transduction family, include a small number of polypeptides: Gi alpha 1 (GNAI1), Gi alpha 2 (GNAI2), and Gi alpha 3 (GNAI3). A cDNA for the human GNAI2 gene has been isolated from a human T-cell library and is mapped by chromosomal in situ hybridization to the short arm of chromosome 3 at 3p21. A related sequence, GNAI2L, is mapped by in situ hybridization to the short arm of chromosome 12 at p12-p13. These mapping results are further supported by amplification of GNAI2-specific sequences in a monochromosomal human/rodent somatic cell hybrid containing only human chromosome 3. Of note, these assignments are to chromosome regions in which other G proteins reside. Localization of GNAI2 to 3p21 is of great interest as this region of the short arm of chromosome 3 is frequently involved in rearrangements in various human tumors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 12*
  • Chromosomes, Human, Pair 3*
  • DNA
  • GTP-Binding Proteins / genetics*
  • Humans
  • Hybrid Cells
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Peptides / genetics
  • Polymerase Chain Reaction

Substances

  • Peptides
  • DNA
  • GTP-Binding Proteins